Human Mesenchymal Stem Cell Transplantation Improved Functional Outcomes Following Spinal Cord Injury Concomitantly with Neuroblast Regeneration

Purpose: Spinal cord injury (SCI) is damage to the spinal that resulted in irreversible neuronal loss, glial scar formation and axonal injury. Herein, we used human amniotic fluid mesenchymal stem cells (hAF-MSCs) their conditioned medium (CM), investigate ability neuroblast astrocyte production as well functional recovery following SCI. Methods: Fifty-four adult rats were randomly divided into nine groups (n=6), included: Control, SCI, (SCI + DMEM), CM), MSCs), Astrocyte), Astrocyte+ CM) MSCs). Following laminectomy SCI induction, DMEM, CM, MSCs, Astrocytes injected. Wester-blot was performed explore levels of Sox2 protein MSCs-CM. The immunofluorescence staining against DCX GFAP done. Finally, Basso-Beattie-Brenham (BBB) locomotor test conducted assess neurological outcomes. Results: Our results showed MSCs increased number endogenous DCX-positive decreased GFAP-positive by mediating juxtacrine paracrine mechanisms (p<.001). Transplanted astrocytes converted neuroblasts rather than under influence CM Moreover, indexes promoted those received CM. Conclusion: Taken together, our data indicate via pathways could direct neural (NSCs) lineage which indicates capability increasing decline.